麻豆传媒

Inflammation Lab Protocols

The procedures below are common in our lab, and they are specific to our experiments and experiences.  New applications should always be optimized.

Bone marrow preparation

Fc蔚RI staining (fluorescent) for the Countess FLII

Fc蔚RI crosslinking of bone marrow-derived mast cells

General immortalized mammalian cell culture (adherent)

Preparation of cell cultures for surface target flow cytometry

Preparation of cell cultures for intracellular target flow cytometry

Protein isolation

Protein concentration measurement

Western Blotting

Detecting Western blot chemiluminescence using a VersaDoc